Journal
ANNALS OF BIOMEDICAL ENGINEERING
Volume 38, Issue 6, Pages 1977-1988Publisher
SPRINGER
DOI: 10.1007/s10439-010-0035-1
Keywords
Temperature-responsive surface; Extra cellular matrix; N-isopropylacrylamide; Cell attachment; Cell detachment; Polymeric thin surface
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Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
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Temperature-responsive intelligent surfaces, prepared by the modification of an interface mainly with poly(N-isopropylacrylamide) and its derivatives, have been investigated. Such surfaces exhibit temperature-responsive hydrophilic/hydrophobic alterations with external temperature changes, which, in turn, result in thermally modulated attachment and detachment with cells. The advantage of this system is that cells cultured on such temperature-responsive surfaces can be recovered as single cells and/or confluent cell sheets, while keeping the deposited extracellular matrix intact, simply by lowering the temperature without conventional enzymatic treatment. Here, we focus and compare various methods of producing temperature-responsive surfaces for controlling cell attachment/detachment. Spontaneous cell attachment and detachment using several types of temperature-responsive surfaces are mentioned and various effects, such as film thickness and polymer conformation, are discussed. In addition, the development of the next generation of temperature-responsive surfaces using modifications of the polymer coating to allow for rapid cell recovery is summarized.
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