4.4 Article

Expression profile of amylolytic genes in Aspergillus nidulans

Journal

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 70, Issue 10, Pages 2363-2370

Publisher

TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.50694

Keywords

amylase; Aspergillus nidulans; AmyR; CreA; carbon starvation

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Aspergillus nidulans possessed 16 putative amylolytic genes consisting of 7 alpha-glucosidase (agdA-F), 7 alpha-amylase (amyA-F), and 2 glucoamylase (glaA and B) genes on the genome. Among them, the agdA, agdB, agdE, agdF, ainyA, amyB, amyF, and glaB genes were induced by isomaltose. AmyR, a Zn(II)(2)Cys(6) transcription factor, was required for the induction. The isomaltose-inducible genes possessed at least one consensus sequence for AmyR binding, 5'-CGGN(8)CGG, on each promoter region. None of the amylolytic genes was induced by maltose.

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