Dynamics and origin of the non-hydrolysable organic fraction in a forest and a cultivated temperate soil, as determined by isotopic and microscopic studies

We isolated the non-hydrolysable macromolecular organic fraction (insoluble fraction resistant to drastic laboratory hydrolyses) from a temperate, loamy, forest soil (Lacadee, France) and from the soil of an adjacent plot cleared 35 years ago and continuously cropped with maize. The quantitative, morphological (light, scanning and transmission electron microscopy) and isotopic (bulk delta C-13, individual compound delta C-13 and radiocarbon dating) features of these two non-hydrolysable fractions were determined and compared. It appeared that: (i) extensive degradation of the non-hydrolysable material inherited from the forest soil occurred upon cropping, revealing that its resistance to drastic laboratory hydrolyses is not paralleled by a great resistance to natural biodegradation triggered by change in land use; (ii) only small inputs of maize-derived compounds occurred in the non-hydrolysable fraction of the cultivated soil so that, in spite of extensive degradation, the forest-inherited carbon still predominates; (iii) the non-hydrolysable fractions of both soils comprise the same components (wood debris, spores, pollen, and, predominantly, granular organic aggregates), which correlate with the previously identified chemical components (charcoal, aliphatic lipid components and melanoidin-like components); (iv) the non-hydrolysable fraction of the cropped soil shows a greater contribution of aliphatic moieties, reflecting differential degradation of the components of the non-hydrolysable material inherited from the forest soil; (v) this degradation resulted in enrichment in the oldest components; and (vi) no relationship is observed, in the two Lacadee soils, between resistance to drastic laboratory hydrolyses, on the one hand, and stability towards biodegradation in situ, on the other. These observations, added to recent ones on other types of soils, suggest that such a conspicuous uncoupling between non-hydrolysable and stable carbon is probably a general feature of organic matter in soil as opposed to sedimentary organic matter.