Journal
JOURNAL OF BIOMOLECULAR SCREENING
Volume 11, Issue 7, Pages 773-781Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/1087057106292138
Keywords
bead-based/immunofluorescence-assay; suspension-array; aptamers; high-throughput screening (HTS); xMAP technology
Ask authors/readers for more resources
Most applications of xMAP (TM) (Luminex (R)) bead-based assay technology in diagnostics and drug discovery use immobilized antigens or antibodies. Here the authors describe the development of novel assay systems in which synthetic oligonucleotides that specifically bind and inhibit other biomolecules-so-called aptamers-are directly immobilized on beads. The robustness, specificity, and sensitivity of aptamer-based assays were demonstrated in a test system that detected human alpha-thrombin in serum samples. xMAP technology was also adapted to competitive screening formats where an aptamer/protein complex was disrupted by a functionally analogous competitor. The results indicate that such assays are excellently suited for diagnostic applications or drug screening, where aptamers serve as competitive binding probes for the identification of small-molecule hits. These methods should be transferable to a large number of applications because specific aptamers can be rapidly generated for almost any protein target.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available