Journal
JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY
Volume 27, Issue 4, Pages 319-329Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15321810600861993
Keywords
horse F(ab)(2) antibody; fractionation protocols; ammonium sulfate; caprylic acid; impurities; anti complementary
Ask authors/readers for more resources
To date, animal derived therapeutic antibodies represent the best and only choice source of antitoxins, especially in developing countries. Furthermore, this industry needs to develop a production protocol to achieve safer products. Recently, several laboratories changed their production protocol from ammonium sulfate (AS) protocol to caprylic acid (CA) fractionation. Our results showed that using the CA protocol leads to improvement in the product quality, as assessed by the albumin and protein content decrease (from 4.75 to 3.54 g/dL and 0.64 to 0.18 g/dL, respectively), which yielded a purer antitoxin product. The F(ab)(2) protein aggregate formation and turbidity have been significantly reduced, 4.60 versus 2.55 and 0.046 versus 0.021 (p < 0.01), respectively. However, the anti-complementary activity was also reduced, from 42 to 33. The total IgG content was higher in CA fractionated products than AS materials. The endotoxin content was worrisome in some F(ab)(2) products.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available