Nitric oxide protects against oxidative stress under heat stress in the calluses from two ecotypes of reed

Calluses from two ecotypes of reed (Phragmites communis Trin.) plant (dune reed [DR] and swamp reed [SR]), which show different sensitivity to heat stress, were used to study plant acclimations to heat stress. SR callus suffered more oxidative damage than DR callus, and DR callus maintained higher relative growth rate and cell viability and lower ion leakage than SR callus under heat stress. Application of two nitric oxide donors, sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine (SNAP), dramatically alleviated heat stress induced ion leakage increase, growth suppression and cell viability decrease in both calluses under beat stress. H2O2 and MDA contents were decreased and the activities of superoxide dismutase, catalase, ascorbate peroxidase and peroxidase increased in both calluses in the presence of NO donors under heat stress. The potassium salt of 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO), a specific NO scavenger, arrested NO donors mediated protective effects. Moreover, measurement of the rate of NO release showed that NO production increased significantly in DR callus while kept stable in SR callus under heat stress. Pretreatment with cPTIO had no effect on SR callus under heat stress while it significantly enhanced oxidative stress in DR callus compared with that of heat stress alone. These results suggest that NO can effectively protect both calluses from oxidative stress induced by heat stress and that NO might act as a signal in activating active oxygen scavenging enzymes under heat stress and thus confer DR callus thermotolerance. (c) 2006 Elsevier Ireland Ltd. All rights reserved.