Journal
FEBS LETTERS
Volume 580, Issue 22, Pages 5385-5391Publisher
WILEY
DOI: 10.1016/j.febslet.2006.09.003
Keywords
JNK; TGF-beta 1; uPA; keratinocytes; EMT
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In this study we analyzed the role of the c-Jun N-terminal kinases (JNK) pathway in the TGF-beta 1 stimulation of urokinase-type plasminogen activator (uPA), initial stages of epithelial-mesenchymal transdifferentiation (EMT) and cell migration. TGF-beta 1 induces JNK phosphorylation, c-Jun transactivation and AP1 activation. The involvement of JNK was evaluated using dominant negative mutants SEK-1 AL, JNK and cJun, depletion of JNK1,2 proteins by treatment of cells with antisense oligonucleotides, as well as the chemical inhibitor SP600125. Our results demonstrated that the JNK pathway is required in the TGF beta 1 enhancement of uPA, fibronectin, E-cadherin delocalization, actin re-organization and vimentin expression, concomitant with the induction of cell migration. These results allow us to suggest a role of JNK in the TGF-beta 1 induction of EMT in relation with the stimulation of malignant properties of mouse transformed keratinocytes. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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