Bisandrographolide from Andrographis paniculata activates TRPV4 channels

Many transient receptor potential ( TRP) channels are activated or blocked by various compounds found in plants; two prominent examples include the activation of TRPV1 channels by capsaicin and the activation of TRPM8 channels by menthol. We sought to identify additional plant compounds that are active on other types of TRP channels. Wescreenedalibraryofextractsfrom50Chinese herbal plants using a calcium-imaging assay to find compounds active on TRPV3 and TRPV4 channels. An extract from the plant Andrographis paniculata potently activated TRPV4 channels. The extract was fractionated further, and the active compound was identified as bisandrographolide A ( BAA). We used purified compoundtocharacterizetheactivityofBAAoncertainTRPVchannel subtypes. Although BAA activated TRPV4 channels with an EC50 of 790 - 950 nM, it did not activate or block activation of TRPV1, TRPV2, or TRPV3 channels. BAA activated a large TRPV4- like current in immortalized mouse keratinocytes ( 308 cells) that have been shown to express TRPV4 protein endogenously. This compound also activated TRPV4 currents in cell- free outside- out patches from HEK293T cells overexpressing TRPV4 cDNA, suggesting that BAA can activate the channel in a membrane- delimited manner. Another related compound, andrographolide, found in abundance in the plant Andrographis was unable to activate or block activation of TRPV4 channels. These experiments show that BAAactivates TRPV4 channels, and we discuss the possibility that activation ofTRPV4byBAAcould play a role in some of the effects of Andrographis extract described in traditional medicine.