Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1129, Issue 2, Pages 262-272Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2006.06.098
Keywords
gas chromatography; beeswax chemical composition; sample preparation; derivatization
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New and simpler methods of sample preparation to determine several families of compounds in beeswax by conventional and high temperature gas chromatography are proposed. To analyze hydrocarbons and palmitates, a dilution of sample is enough whereas for the total acid content, a hydrolysis and simultaneous methylation with BF3-methanol results more effective than the usual methods; for the total content of alcohols, a further acetylation with acetic anhydride is necessary. Free alcohols are directly acetylated in a sample dissolution but for free acids and monoesterified 1,2,3-propanetriols analysis, a previous extraction with acetonitrile is required. The concentrations of all the compounds studied are expressed in weight percentage referred only to one standard: octadecyl octadecanoate. The precision of the analytical methods has been evaluated showing its importance in the analysis of beeswaxes used in apiculture. (c) 2006 Elsevier B.V. All rights reserved.
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