Journal
JOURNAL OF PROTEOME RESEARCH
Volume 5, Issue 10, Pages 2839-2848Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr060328c
Keywords
intrinsically unstructured proteins; disordered proteins; proteomics; mammalian proteome; heat denaturation
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Funding
- NCI NIH HHS [R21CA104568, R01CA092035, R01CA082491, CA21765] Funding Source: Medline
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Intrinsically unstructured proteins (IUPs) represent an important class of proteins primarily involved in cellular signaling and regulation. The aim of this study was to develop methodology for the enrichment and identification of IUPs. We show that heat treatment of NIH3T3 mouse fibroblast cell extracts at 98 C selects for IUPs. The majority of these IUPs were cytosolic or nuclear proteins involved in cell signaling or regulation. These studies represent the first large-scale experimental investigation of the intrinsically unstructured mammalian proteome.
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