Journal
NEUROSCIENCE LETTERS
Volume 406, Issue 3, Pages 165-168Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2006.06.067
Keywords
DJ-1; oxidative stress; cysteine oxidation
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DJ-1 was initially identified by us as a novel oncogene and has recently been found to be a causative gene for familial Parkinon's disease (PD) PARK7. DJ-1 plays roles in transcriptional regulation and in oxidative stress function, and its oxidative state at cysteine residues determines activities of DJ-1. In this study, we found that recombinant DJ-1 expressed in and purified from E. coli was specifically cleaved between glycine and proline at amino acid numbers 157 and 158, respectively, by treatment of DJ-1 with H2O2. A substitution mutant of DJ-1 from cysteine to serine at amino acid number 106, a major oxidation site of DJ-1, was found not to be cleaved under an oxidative condition, suggesting oxidation-dependent cleavage of DJ-1. Cleavage of DJ-1 was also observed in human SH-SY5Y cells that had been treated with H2O2. These results suggest that oxidative stress-induced cleavage of DJ-1 regulates functions of DJ-1. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
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