Journal
YEAST
Volume 23, Issue 13, Pages 921-928Publisher
JOHN WILEY & SONS LTD
DOI: 10.1002/yea.1422
Keywords
primer sequence; Sz. pombe; gene deletion; gene expression; gene tagging; microarray; quantitative PCR
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Funding
- Cancer Research UK [A6517] Funding Source: Medline
- Wellcome Trust [077118] Funding Source: Medline
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PCR-based gene targeting is a popular method for manipulating yeast genes in their normal chromosomal locations. The manual design of primers, however, can be cumbersome and error-prone. We have developed a straightforward web-based tool that applies user-specified inputs to automate and simplify the task of primer selection for deletion, tagging and/or regulated expression of genes in Schizosaccharomyces pombe. This tool, named PPPP (for Pombe PCR Primer Programs), is available at http://www.sanger.ac.uk/PostGenomics/S-pombe/software/. We also present a searchable Microarray Primer Database to retrieve the sequences and accompanying information for primers and PCR products used to build our in-house Sz. pombe microarrays. This database contains information on both coding and intergenic regions to provide context for the microarray data, and it should be useful also for other applications, such as quantitative PCR. The database can be accessed at http://www.sanger.ac.uk/PostGenomics/S-pombe/microarray/. Copyright (c) 2006 John Wiley & Sons, Ltd.
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