Journal
FEBS LETTERS
Volume 580, Issue 24, Pages 5690-5696Publisher
WILEY
DOI: 10.1016/j.febslet.2006.09.020
Keywords
PKA; AKAP proteins; cardiomyocytes
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Evidence showing the existence in the inner compartment of rat-heart mitochondria of AKAP121 and associated PKA is presented. Immunoblotting analysis and trypsin digestion pattern show that 90% or more of mitochondrial C-PKA, R-PKA and AKAP121 is localized in the inner mitochondrial compartment, when prepared both from isolated mitochondria or cardiomyocyte cultures. This localization is verified by measurement of the specific catalytic activity of PKA, radiolabelling of R-PKA by (32)p-phosphorylated C-PKA and of AKAP by P-32-phosphorylated R-PKA and electron microscopy of mitochondria exposed to gold-conjugated AKAP121 antibody. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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