Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 103, Issue 43, Pages 15945-15950Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0607528103
Keywords
dendritic cells; live cell imaging; lysosome; phagocytosis; yeast
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Funding
- NCI NIH HHS [1F32CA105862-01, F32 CA105862] Funding Source: Medline
- NIAID NIH HHS [K08 AI057999, 5K08AI57999, 5R01AI034893-13, R01 AI034893] Funding Source: Medline
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The subcellular localization of the cluster of differentiation 63 (CD63) tetraspanin and its interaction with the class II MHC antigen presentation pathway were examined in the context of phagocytosis by live cell imaging, by using monomeric red fluorescent protein-tagged mouse CD63 expressed in primary bone marrow-derived cell cultures. Upon phagocytosis of Cryptococcus neoformans and polystyrene beads, CD63 was recruited selectively to C neoformans-containing phagosomes in a MyD88-independent acidification-dependent manner. Bead-containing phagosomes, within a C neoformans-containing cell, acidified to a lesser extent and failed to recruit CD63 to a level detectable by microscopy. CD63 recruitment to yeast phagosomes occurred independently of class 11 MHC and LAMP-1. These observations indicate that the composition of distinct phagosomal compartments within the same cell is determined by phagosomal cargo and may affect the outcome of antigen processing and presentation.
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