Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1131, Issue 1-2, Pages 281-284Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2006.07.095
Keywords
phenyllactic acid determination; HPLC; lactic acid bacteria (LAB)
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Phenyllactic acid (PLA) is an organic acid produced by some strains of lactic acid bacteria (LAB) and concentrations higher than 7.5 mg/ml inhibit growth of moulds and yeasts. Since PLA can be used to select LAB, a rapid, simple and cheap method for its determination is desirable. Typical methods for its analysis in broth are time-consuming, analytically complicated, and have poor recoveries. Herein we propose a simple and rapid method that does not require extraction, but only microfiltration of broth before injection in HPLC. The improved chromatographic conditions allow separation and quantification of PLA with a recovery of 98.7%. The method is highly reproducible with an intraday repeatability of the total peak area of 2.00%, while an interday repeatability of 2.69%. (c) 2006 Elsevier B.V. All rights reserved.
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