Journal
FEBS LETTERS
Volume 580, Issue 25, Pages 5869-5874Publisher
WILEY
DOI: 10.1016/j.febslet.2006.09.053
Keywords
vascular endothelial growth factor; human embryonic stem cells; differentiation; embryonic germ layers; neurons
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Vascular endothelial growth factor (VEGF), a potent mitogen for vascular endothelial cells, has been suggested as a modulator that is involved in neurogenesis as well as angiogenesis. Here, we directly examined the effect of VEGF on neuroectodermal differentiation using human embryonic stem cells (hESCs). VEGF treatment upregulated the expression of neuroectodermal genes (SoxI and Nestin) during germ layer formation in embryoid bodies (EBs) and efficiently increased the number of neural rosettes expressing both Pax6 and Nestin. The neural progenitors generated from VEGF-treated EBs further differentiated into cells that showed a similar pattern of gene expression observed in the development of dopaminergic neurons upon terminal differentiation. These results support the neurogenic effect of VEGF on hESC differentiation. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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