4.5 Article

Genome wide cDNA-AFLP analysis of genes rapidly induced by combined sucrose and ABA treatment in rice cultured cells

Journal

FEBS LETTERS
Volume 580, Issue 25, Pages 5947-5952

Publisher

WILEY
DOI: 10.1016/j.febslet.2006.09.065

Keywords

ABA; sucrose; AGPase; starch; cDNA-AFLP

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We identified 27 genes induced by combined sucrose and ABA treatment from rice cultured cells with cDNA-AFLP. Thirteen of these up-regulated genes were induced 30 min after the co-treatment. This suite of genes includes starch biosynthesis related genes. Type A genes were expressed only in the presence of both sucrose and ABA. Type B genes were expressed in the presence of sucrose or ABA and the expression was dramatically enhanced by the co-treatment of sucrose and ABA. These results indicate that multiple steps of starch biosynthesis and other processes may be regulated by at least two different pathways. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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