Journal
FEBS LETTERS
Volume 580, Issue 25, Pages 5894-5898Publisher
WILEY
DOI: 10.1016/j.febslet.2006.09.055
Keywords
ATP; DIDS; synaptic vesicles; transport
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ATP is an excitatory neurotransmitter in the central and peripheral nervous system. We investigated ATP accumulation in highly purified brain synaptic vesicles (SVs). Based on the amount of ATP accumulated in SVs under the conditions used, ATP is not transported against a concentration gradient but rather appears to have a Delta mu H+-independent mechanism. ATP transport was inhibited by DIDS and NEM, but was not affected by Mg2+ or by pre-incubation with nucleotides. These results suggest a unique transport mechanism that does not involve exchange with other nucleotides or protons, unlike other known neurotransmitter transport systems. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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