Journal
BIOLOGICAL & PHARMACEUTICAL BULLETIN
Volume 29, Issue 11, Pages 2313-2316Publisher
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.29.2313
Keywords
Phyllanthus emblica; euphorbiaceae; random amplified polymorphic DNA (RAPD); sequence characterized amplified region (SCAR)
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Correct genotype identification of medicinal plant material remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. The present study was carried out to develop DNA based marker for identification of Phyllanthus emblica LINN. A putative marker (1.1 kb) specific for P. emblica was identified by Random Amplified Polymorphic DNA (RAPD) technique. Sequence Characterized Amplified Region (SCAR) marker was developed from the RAPD amplicon. The SCAR marker was found useful for identification of P. emblica in its commercial samples and Triphalachurna, a multi-component Ayurvedic formulation.
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