Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 72, Issue 11, Pages 7422-7426Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01550-06
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We show here that the paaABCDE genes of the paa cluster responsible for phenylacetate degradation in Escherichia coli W encode a five-component oxygenase that hydroxylates phenylacetyl-coenzyme A (CoA), the first intermediate of the pathway. The primary structure of the subunits of bacterial phenylacetyl-CoA oxygenases revealed that these enzymes constitute the prototype of a new and distinct group of the large bacterial diiron multicomponent oxygenase family.
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