Journal
NATURE STRUCTURAL & MOLECULAR BIOLOGY
Volume 13, Issue 11, Pages 1002-1009Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb1156
Keywords
-
Funding
- NIGMS NIH HHS [R01-GM073863, R01 GM070521, R01 GM073863-03, R01 GM073863-01, R01 GM073863-02, R01 GM073863-04, R01 GM073863, R01-GM70521] Funding Source: Medline
Ask authors/readers for more resources
The Ro autoantigen is ring-shaped, binds misfolded noncoding RNAs and is proposed to function in quality control. Here we determine how Ro interacts with misfolded RNAs. Binding of Ro to misfolded precursor (pre)-5S ribosomal RNA requires a single-stranded 3' end and helical elements. As mutating most sequences of the helices and tail results in modest decreases in binding, Ro may be able to associate with a range of RNAs. Ro binds several other RNAs that contain single-stranded tails. A crystal structure of Ro bound to a misfolded pre-5S rRNA fragment reveals that the tail inserts into the cavity, while a helix binds on the surface. Most contacts of Ro with the helix are to the backbone. Mutagenesis reveals that Ro has an extensive RNA-binding surface. We propose that Ro uses this surface to scavenge RNAs that fail to bind their specific RNA-binding proteins.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available