Journal
PHOTOCHEMISTRY AND PHOTOBIOLOGY
Volume 82, Issue 6, Pages 1634-1637Publisher
WILEY
DOI: 10.1562/2006-01-12-RN-774
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Funding
- NCI NIH HHS [P01 CA066081-069001, CA-66081, P01 CA066081-060003, R01 CA081090-04, R01 CA084462-04, P01 CA066081, CA81090, R01 CA084462, CA-84462] Funding Source: Medline
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Singlet oxygen is a highly reactive electrophilic species that reacts rapidly with electron-rich moieties, such as the double bonds of lipids, thiols, and ascorbate (AscH(-)). The reaction of ascorbate with singlet oxygen is rapid (k = 3 X 10(8) M-1 s(-1)). Here we have investigated the stoichiometry of this reaction. Using electrodes to make simultaneous, real-time measurements of oxygen and hydrogen peroxide concentrations, we have investigated the products of this reaction. We have demonstrated that hydrogen peroxide is a product of this reaction. The stoichiometry for the reactants of the reaction (1(1)O(2) + 1AscH(-) -> 1H(2)O(2) + 1dehydroascorbic) is 1:1. The formation of H2O2 results in a very different oxidant that has a longer lifetime and much greater diffusion distance. Thus, locally produced singlet oxygen with a half-life of 1 ns to 1 mu s in a biological setting is changed to an oxidant that has a much longer lifetime and thus can diffuse to distant targets to initiate biological oxidations.
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