Journal
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
Volume 29, Issue 5-6, Pages 335-343Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.cimid.2006.09.004
Keywords
Aeromonas salmonicida; phagocytosis; leukocytes; rainbow trout; probiotics
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A method is described for the rapid and sensitive assay of phagocytosis that utilizes radioactively labelled bacteria. With this method, we observed that phagocytosis of heat-inactivated Aeromonas salmonicida by leukocytes isolated from gut of rainbow trout fed with different viable probiotics (Lactococcus lactis subsp. lactis, Lactobacillus sakei, and Leuconostoc mesenteroides) was significantly higher (P < 0.05) after 2 weeks of probiotic-feeding than the control group. However, only phagocytosis of live A. salmonicida by the leukocytes isolated from gut of rainbow trout fed with L. lactis subsp. lactis was significantly higher (P < 0.05) than the control group. (c) 2006 Elsevier Ltd. All rights reserved.
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