Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 386, Issue 6, Pages 1727-1733Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-006-0767-9
Keywords
ruscogenin; immunoassay; pharmacokinetics; biological sample; crude Chinese medicine
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Ruscogenin is a major bioactive steroidal aglycone found in the Chinese medicine, Ophiopogon japonicus. We have developed a quantitative determination of ruscogenin with an indirect enzyme-linked immunosorbent (ELISA) assay using polyclonal antibodies against ruscogenin conjugated with bovine serum albumin. This assay was highly sensitive, and it had considerably less cross-reactivity to diosgenin and sarsasapogenin, but high cross-reactivity to ruscogenin glycosides. The assay was successfully used for the measurement of ruscogenin concentrations in crude Chinese medicines and in biological samples from a pharmacokinetics study of ruscogenin.
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