Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 386, Issue 5, Pages 1367-1378Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-006-0707-8
Keywords
Brassica juncea (Indian mustard); Heliantus annuus (sunflower); water-soluble selenium-containing proteins; HPLC/ICP-MS
Funding
- NIEHS NIH HHS [ES 04908] Funding Source: Medline
Ask authors/readers for more resources
Selenoproteins have been identified in a diverse range of organisms, including bacteria and animals. Their occurrence and role in the plant kingdom are, however, less well-understood. This work investigated the water-soluble selenium-containing proteins extracted from a selenium-accumulating plant species (Brassica juncea) and a nonaccumulator species (Helianthus annuus) exposed to varying forms and concentrations of selenium. Firstly, protein extracts were analyzed by size exclusion chromatography coupled to inductively coupled plasma mass spectrometry; specific detection was achieved by monitoring characteristic isotopes. Then, proteolytic digests of the plant extracts were analyzed by reversed phase chromatography coupled to ICP-MS in order to investigate selenoamino acid and selenopeptide content. Selenomethionine was observed to be the primary constituent of the proteins of the nonaccumulator plant, while selenocystine and selenomethionine were found in the same proportion in the accumulator extract. One main selenium-containing species was present at higher levels in the root digests than in the leaf digests; levels were greater in the nonaccumulator than in the accumulator plant.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available