Journal
BIOTECHNOLOGY LETTERS
Volume 28, Issue 22, Pages 1841-1847Publisher
SPRINGER
DOI: 10.1007/s10529-006-9168-6
Keywords
DHA biosynthesis genes; docosahexaenoic acid; eicosapentaenoic acid; Moritella marina strain MP-1; polyunsaturated fatty acids
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The docosahexaenoic acid (DHA) biosynthesis gene cluster (pDHA3) from the DHA-producing Moritella marina strain MP-1 includes the genes pfaA, pfaB, pfaC, and pfaD, which are similar to the genes of polyketide biosynthesis. When this cluster was co-expressed in Escherichia coli with M. marina MP-1 pfaE, which encodes phosphopantetheinyl transferase, DHA was biosynthesized. The maximum production of DHA (5% of total fatty acids) was observed at 15 C. This is the first report of the recombinant production of DHA in a polyketide biosynthesis mode.
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