Journal
ANALYTICAL CHEMISTRY
Volume 78, Issue 21, Pages 7577-7581Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac061451q
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Funding
- NIBIB NIH HHS [R01 EB002044, R15 EB016870, R01 EB002044-06A1] Funding Source: Medline
- NIGMS NIH HHS [R01 GM002044] Funding Source: Medline
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The nucleophilic addition of the aminothiols homocysteine (HCY), cysteine (CYS), and glutathione (GSH) to the electrogenerated quinone of fluorone black (1) via the ECE mechanism is reported. It is demonstrated that 1 selectively reacts with GSH to form the bis-GSH adduct, 1-(GSH)(2), while only the monothiol adducts were generated in the presence of HCY and CYS (1-HCY and 1-CYS, respectively). The more anodic E-pa of 1-(GSH)(2) relative to 1 and 1-GSH (Delta E-pa similar to +0.14) is the voltammetric signature that allows the discrimination of GSH from HCY and CYS. It is also shown that the presence of structurally similar aminothiolss-HCY and CYS-posed no interference to the signature voltammetric response of 1-(GSH)(2).
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