Journal
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR
Volume 85, Issue 3, Pages 562-568Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.pbb.2006.10.010
Keywords
ethanol self-administration; water deprivation; nucleus accumbens; gelatin; polycose
Funding
- NIAAA NIH HHS [R01 AA014708, AA014708] Funding Source: Medline
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A novel procedure for initiation of voluntary ethanol consumption in the rat was evaluated in terms of ease of initiation, consistency, and resulting brain ethanol levels. The jello shot consists of 10% ethanol in gelatin along with a caloric source (Polycose). Initiation of jello shot consumption in Sprague-Dawley rats required no food or water restriction and resulted in initial daily (8.4 +/- 0.6 g/kg body weight) and eventual hourly (11 +/- 0.1 g/kg body weight) intake of ethanol comparable to other procedures using either alcohol-preferring or non-genetically selected rats. Rat intake of ethanol via jello shots recovered quickly from environmental alterations and surgical implantation of a guide cannula. During 1-h free access sessions, consumption of the jello shot occurred during the initial 10 min and resulted in a dose-related increase in ethanol levels in nucleus accumbens measured using microdialysis. These brain ethanol levels were comparable to those achieved using other self-administration methods. However, when 0.5 g/kg ethanol was gavaged either in jello shot or saline, there was about a 20% decrease in brain ethanol concentrations after gavage of the jello shot compared to saline. Even so, lack of a need for initial food or water deprivation and the rapidity with which stable self-administration can be achieved both suggest utility of the jello shot as a completely voluntary ethanol procedure. (c) 2006 Elsevier Inc. All rights reserved.
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