Journal
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
Volume 291, Issue 5, Pages H2473-H2482Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.00172.2006
Keywords
reactive oxygen species; peroxides; sulfhydryl compounds; iberiotoxin; delayed rectifier potassium channels; coronary circulation
Funding
- NCRR NIH HHS [P20 RR018766] Funding Source: Medline
- NHLBI NIH HHS [HL-67804, HL-65203, HL-32788, HL-73755] Funding Source: Medline
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Hydrogen peroxide (H2O2) is a proposed endothelium-derived hyperpolarizing factor and metabolic vasodilator of the coronary circulation, but its mechanisms of action on vascular smooth muscle remain unclear. Voltage-dependent K+ (K-V) channels sensitive to 4-aminopyridine (4-AP) contain redox-sensitive thiol groups and may mediate coronary vasodilation to H2O2. This hypothesis was tested by studying the effect of H2O2 on coronary blood flow, isometric tension of arteries, and arteriolar diameter in the presence of K+ channel antagonists. Infusing H2O2 into the left anterior descending artery of anesthetized dogs increased coronary blood flow in a dose-dependent manner. H2O2 relaxed left circumflex rings contracted with 1 mu M U46619, a thromboxane A(2) mimetic, and dilated coronary arterioles pressurized to 60 cmH(2)O. Denuding the endothelium of coronary arteries and arterioles did not affect the ability of H2O2 to cause vasodilation, suggesting a direct smooth muscle mechanism. Arterial and arteriolar relaxation by H2O2 was reversed by 1 mM dithiothreitol, a thiol reductant. H2O2-induced relaxation was abolished in rings contracted with 60 mM K+ and by 10 mM tetraethylammonium, a nonselective inhibitor of K+ channels, and 3 mM 4-AP. Dilation of arterioles by H2O2 was antagonized by 0.3 mM 4-AP but not 100 nM iberiotoxin, an inhibitor of Ca2+-activated K+ channels. H2O2-induced increases in coronary blood flow were abolished by 3 mM 4-AP. Our data indicate H2O2 increases coronary blood flow by acting directly on vascular smooth muscle. Furthermore, we suggest 4-AP-sensitive K+ channels, or regulating proteins, serve as redox-sensitive elements controlling coronary blood flow.
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