Journal
AFRICAN JOURNAL OF MARINE SCIENCE
Volume 28, Issue 3-4, Pages 719-721Publisher
NATL INQUIRY SERVICES CENTRE PTY LTD
DOI: 10.2989/18142320609504221
Keywords
abalone; DNA extraction; DNA quality; epipodial tentacles; mucus; non-destructive sampling; PCR
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Good-quality biological material is needed to obtain intact deoxyribonucleic acid (DNA) for use in molecular techniques such as the polymerase chain reaction (PCR). Non-destructive sampling protocols of juvenile abalone Haliotis midae (7-15 months old) were tested in order to collect material for DNA extraction. DNA was successfully extracted from epipodial tentacles and mucus samples. PCR results confirmed the good quality of the DNA and the reliability of the method.
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