Journal
PLANT CELL REPORTS
Volume 25, Issue 11, Pages 1213-1218Publisher
SPRINGER
DOI: 10.1007/s00299-006-0186-6
Keywords
Glycine max; RNA silencing; SbDV; transformation; resistance
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In an attempt to generate soybean plants resistant to soybean dwarf virus (SbDV), we transformed a construct containing inverted repeat-SbDV coat protein (CP) genes spaced by beta-glucuronidase (GUS) sequences into soybean somatic embryos via microprojectile bombardment. Three T-0 plants with an introduced CP gene were obtained, and one generated T-1 seeds. The presence of the transgene in T-1 plants was confirmed by PCR and Southern blot hybridization analysis, but expression of CP was not detected by northern blot hybridization analysis. Two months after inoculation of SbDV by aphid, T-2 plants contained little SbDV-specific RNA and remained symptomless. These plants contained SbDV-CP-specific siRNA. These results suggest that the T-2 plants achieved resistance to SbDV by an RNA-silencing-mediated process.
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