Journal
JOURNAL OF VIROLOGY
Volume 80, Issue 21, Pages 10514-10521Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00485-06
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Funding
- Biotechnology and Biological Sciences Research Council [BB/D003296/1, BBS/E/R/00000684] Funding Source: researchfish
- BBSRC [BB/D003296/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/R/00000684, BB/D003296/1] Funding Source: Medline
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We used a porcine microarray containing 2,880 cDNAs to investigate the response of macrophages to infection by a virulent African swine fever virus (ASFV) isolate, Malawi LIL20/1. One hundred twenty-five targets were found to be significantly altered at either or both 4 h and 16 h postinfection compared with targets after mock infection. These targets were assigned into three groups according to their temporal expression profiles. Eighty-six targets showed increased expression levels at 4 h postinfection but returned to expression levels similar to those in mock-infected cells at 16 h postinfection. These encoded several proinflammatory cytokines and chemokines, surface, proteins, and proteins involved in cell signaling and trafficking pathways. Thirty-four targets showed increased expression levels at 16 h postinfection compared to levels at 4 It postinfection and in mock-infected cells. One host gene showed increased expression levels at both 4 and 16 h postinfection compared to levels in mock-infected cells. The microarray results were validated for 12 selected genes by quantitative real-time PCR. Levels of protein expression and secretion were measured for two proinflammatory cytokines, interleukin 1 beta and tumor necrosis factor alpha, during a time course of infection with either the virulent Malawi LIL20/1 isolate or the OUR T88/3 nonpathogenic isolate. The results revealed differences between these two ASFV isolates in the amounts of these cytokines secreted from infected cells.
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