Journal
JOURNAL OF VIROLOGY
Volume 80, Issue 22, Pages 11082-11093Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.01307-06
Keywords
-
Categories
Funding
- Medical Research Council [G0400802, G9818340B] Funding Source: researchfish
- MRC [G0400802] Funding Source: UKRI
- Medical Research Council [G0400802] Funding Source: Medline
- NCI NIH HHS [R01 CA108304, CA108304] Funding Source: Medline
- NIAID NIH HHS [R01 AI050798, AI50798] Funding Source: Medline
Ask authors/readers for more resources
The virological and cellular consequences of persistent hepatitis C virus (HCV) infection have been elusive due to the absence of the requisite experimental systems. Here, we report the establishment and the characteristics of persistent in vitro infection of human hepatoma-derived cells by a recently described HCV genotype 2a infectious molecular clone. Persistent in vitro infection was characterized by the selection of viral variants that displayed accelerated expansion kinetics, higher peak titers, and increased buoyant densities. Sequencing analysis revealed the selection of a single adaptive mutation in the HCV E2 envelope protein that was largely responsible for the variant phenotype. In parallel, as the virus became more aggressive, cells that were resistant to infection emerged, displaying escape mechanisms operative at the level of viral entry, HCV RNA replication, or both. Collectively, these results reveal the existence of coevolutionary events during persistent HCV infection that favor survival of both virus and host.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available