Chemoenzymatic ubiquitination of artificial substrates

Probing an alternative route. Ubiquitin and Ub-like proteins (Ubl) are attached to cellular proteins through a conserved readily transferred onto cysteine-containing artificial substrates. This finding provides new insight into the chemistry conjugation pathway. We demonstrate of Ub (Ubl) conjugation and can be here that Ub and Ubl thioesters, the key used for creating different protein intermediates of this pathway, can be probes and microarrays.