Journal
CARBOHYDRATE RESEARCH
Volume 341, Issue 15, Pages 2507-2515Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2006.06.027
Keywords
chitinase; Bacillus subtilis; antifungal; chitin oligosaccharides; antitumor
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Bacillus subtilis W-118, a strain that produces antifungal materials, excreted a chitinase when cultured in a medium containing shrimp- and crab-shell powder as the major carbon source. This chitinase, purified by sequential chromatography, had a molecular mass of 20,600 Da and a pI of 6. The optimum pH optimum temperature, and pH stability of the chitinase were pH 6, 37 degrees C, and pH 5-7, respectively. The unique characteristics of the purified chitinase include low molecular mass and acidic pI. In the investigation of the inhibitory activity, it was found that the growth of Fusarium oxysporum was 100% inhibited after incubation for 1 day with sterilized W-118 chitinase solution (5.6 units/mL). The chitinase hydrolyzates of chitin with low degrees of polymerization (DP 1-6) were analyzed by HPLC. Longer reaction times led to the generation of chitin oligosaccharides with lower DP. The chitin oligosaccharides were examined for their inhibitory effects on F oxysporum and human leukemia cell lines. (c) 2006 Elsevier Ltd. All rights reserved.
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