Journal
JOURNAL OF MOLECULAR STRUCTURE
Volume 799, Issue 1-3, Pages 196-203Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molstruc.2006.03.069
Keywords
FTIR imaging; 2DCOS; human cartilage; concentration gradient; collagen; proteoglycans
Categories
Ask authors/readers for more resources
This paper explores a new application of two-dimensional correlation spectroscopy (2DCOS) in FTIR spectroscopic imaging analysis of biological samples. A particular example demonstrated in this paper is the characterization of concentration gradients of collagen and proteoglycans in human patellar cartilage. A focal plane array detector-based FTIR imaging system has been proven to be an efficient tool to detect early collagen and proteoglycans degradation in developing osteoarthrosis through evaluating compositional changes of osteoarthritic cartilage along the depth. However, the closely overlapped bands of collagen and proteoglycans make normal spectral and spatial analysis difficult. With 2DCOS analysis of the imaging data, it is possible to enhance the spectral resolution and reveal distinctive compositional changes that are normally hidden with conventional approaches. The combined technique, FTIR imaging enhanced with 2DCOS, provides new possibilities to solve challenging problems in the analysis of complex biological systems. (c) 2006 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available