Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 843, Issue 2, Pages 175-182Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2006.05.039
Keywords
Avian embryos; olfactory epithelium; neural stem cells; SdFFF; cell separation
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The aim of the present study was to isolate neural stem cells from a complex tissue: the avian olfactory epithelium; by using sedimentation field flow fractionation (SdFFF). By using Hyperlayer clution mode, fraction collection and cell characterization methods, results shows that SdFFF could be a useful cell sorter to isolate an enriched, viable and sterile immature neural cell fraction from which the reconstitution of a complete epithelium was possible. In culture, SdFFF eluted cells first led to a pseudoplacodal epithelioid cell type from which derived floating cells. These cells were then able to generate neurosphere-like structures which were composed of cell having many features of immature cells: undifferentiated. self-renewable and multipotentiality. Such a population might be used as a model to improve our understanding of the mechanisms of olfactory neoneurogenesis. (c) 2006 Elsevier B.V. All rights reserved.
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