Journal
VACCINE
Volume 24, Issue 44-46, Pages 6647-6650Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2006.06.001
Keywords
viral RNAs; trans-complementation; RNA polymerase II; packaging; vaccine; influenza A virus
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The incorporation of the eight gene segments of influenza A virus in virions, either through a random or a specific mechanism, has been under debate for many years. Using reverse genetics techniques and trans-complementation of viral proteins, we showed that the production of viruses containing seven gene segments is very inefficient, which can be overcome by providing the 8th gene segment in a non-functional form. We conclude that sequences in the 5' and 3' ends of the polymerase gene segments contain packaging signals. Our methods will facilitate the further mapping of the packaging signals of influenza A virus. (c) 2006 Elsevier Ltd. All rights reserved.
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