Journal
JOURNAL OF BIOTECHNOLOGY
Volume 126, Issue 3, Pages 313-324Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2006.05.005
Keywords
Bacillus megaterium; proteome analysis; high cell density; metalloprotease; immune inhibitor A; stress response
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Intracellular and extracellular proteome analysis was carried out by combined two-dimensional gel electrophoresis and mass spectrometric analysis (2DE/MS) for high cell density fed-batch culture of recombinant Bacillus megaterium strains. In the early feeding phase with a constant growth rate of 0.12 h(-1) under glucose limitation, high expression and secretion of a metalloprotease (referred as Bmg1465) was detected. The transient appearance of this metalloprotease was found both as cell-associated and as secreted into the culture medium. Searching homologous proteins for functional assignment led to an unambiguous identification of Bmg1465 as a zinc-binding metalloprotease of the type immune inhibitor A (InhA). Metalloproteases of this type are currently considered as typical virulence factors associated with pathogenic Bacillus species. The result raises questions concerning the intrinsic function(s) of Bmg1465 in B. megaterium, which has the GRAS status, with respect to its stress response in high cell density culture. (c) 2006 Elsevier B.V. All rights reserved.
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