Journal
FEBS LETTERS
Volume 580, Issue 26, Pages 6105-6108Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2006.10.008
Keywords
NDI1; mitochondrial ROS; rotenone; dopaminergic cells; complex I
Funding
- NIDDK NIH HHS [R01DK053244] Funding Source: Medline
- NINDS NIH HHS [R01NS048441] Funding Source: Medline
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Using rat dopaminergic and human neuroblastoma cell lines transduced with the NDI1 gene encoding the internal NADH dehydrogenase (Ndi1) from Saccharomyces cerevisiae, we investigated reactive oxygen species (ROS) generation caused by complex I inhibition. Incubation of non-transduced cells with rotenone elicited oxidative damage to mitochondrial DNA as well as lipid peroxidation. In contrast, oxidative stress was significantly decreased when the cells were transduced with NDI1. Furthermore, mitochondria from the NDI1-transduced cells showed a suppressed rate of ROS formation by the complex I inhibitors. We conclude that the Ndil enzyme is able to suppress ROS overproduction from defective complex I. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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