Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 103, Issue 46, Pages 17160-17163Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0608319103
Keywords
biocomputers; biocatalysis; enzymes
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The assembly of three concatenated enzyme-based logic gates consisting of OR, AND, XOR is described. Four biocatalysts, acetylcholine esterase, choline oxiclase, microperoxidase-11, and the NAD(+)-dependent glucose dehydrogenase, are used to assemble the gates. Four inputs that include acetylcholine, butyrylcholine, O-2, and glucose are used to drive the concatenated-gates system. The cofactor NAD(+), and its reduced 1,4-dihydro form, NADH, are used as a reporter couple, and these provide an optical output for the gates. The modulus of the absorbance changes of NADH is used as a readout signal.
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