Journal
WORLD JOURNAL OF GASTROENTEROLOGY
Volume 12, Issue 42, Pages 6893-6897Publisher
BAISHIDENG PUBLISHING GROUP INC
DOI: 10.3748/wjg.v12.i42.6893
Keywords
suppression subtracted hybridization; liver neoplasm; metastasis suppression genes
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AIM: To identify genes differentially expressed in mouse hepatocarcinoma ascites cell line with low potential of lymphogenous metastasis. METHODS: A subtracted cDNA library of mouse hepatocarcinoma cell line with low potential of lymphogenous metastasis Hca-P and its synogenetic cell line Hca-F with high metastatic potential was constructed by suppression subtracted hybridization (SSH) method. The screened clones of the subtracted library were sequenced and GenBank homology search was performed. RESULTS: Fifteen differentially expressed cDNA fragments of Hca-P were obtained which revealed 8 known genes, 4 expressed sequence tags (ESTs) and 3 cDNAs showed no homology. CONCLUSION: Tumor metastasis is an incident involving multiple genes. SSH is a useful technique to detect differentially expressed genes and an effective method to clone novel genes. (C) 2006 The WJG Press. All rights reserved.
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