Journal
SCIENCE
Volume 314, Issue 5802, Pages 1139-1143Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1131398
Keywords
-
Categories
Funding
- Howard Hughes Medical Institute Funding Source: Medline
- NIGMS NIH HHS [GM41376, R01 GM041376, R01 GM041376-15, R01 GM041376-18, R01 GM041376-16, R01 GM041376-17, R37 GM041376] Funding Source: Medline
Ask authors/readers for more resources
Using single-molecule DNA nanomanipulation, we show that abortive initiation involves DNA scrunching - in which RNA polymerase (RNAP) remains stationary and unwinds and pulls downstream DNA into itself - and that scrunching requires RNA synthesis and depends on RNA length. We show further that promoter escape involves scrunching, and that scrunching occurs in most or all instances of promoter escape. Our results support the existence of an obligatory stressed intermediate, with approximately one turn of additional DNA unwinding, in escape and are consistent with the proposal that stress in this intermediate provides the driving force to break RNAP-promoter and RNAP-initiation-factor interactions in escape.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available