Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 844, Issue 1, Pages 112-118Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2006.07.012
Keywords
s-adenosylmethionine; s-adenosylhomocysteine; pentafluorophenylpropyl; tandem mass spectrometry; mouse embryo
Funding
- MRC [G0401315] Funding Source: UKRI
- Medical Research Council [G0401315] Funding Source: researchfish
- Medical Research Council [G0401315] Funding Source: Medline
- Wellcome Trust [068883] Funding Source: Medline
Ask authors/readers for more resources
The potential importance of the methylation cycle during embryonic development necessitates the establishment of methodology to detect alterations in the relative abundance of s-adenosylmethionine (SAM) and s-adenosylhomocysteine (SAH) in an embryonic experimental system. We have developed a precise and sensitive method for measurement of SAM and SAH based on liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) in single neurulation-stage mouse embryos. Use of a penta-fluorinated high-performance liquid chromatography (HPLC) stationary phase gave enhanced sensitivity due to optimal ionisation in organic mobile phase and increased retention time compared to standard reversed-phase separation. Calibration curves suitable for the analysis of neurulation-stage mouse embryos (SAM 0.02-25.0 mu M, SAH 0.01-10.0 mu M) were linear (r(2) > 0.997) with limits of detection for SAM and SAH of 10 and 2.5 nmol/L, respectively. (c) 2006 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available