Verticilide: Elucidation of absolute configuration and total synthesis

Verticilide (1) is a 24- membered cyclic depsipeptide isolated from the culture broth of Verticillium sp. FKI- 1033. It inhibits ryanodine binding to ryanodine receptor (RyR) and has insecticidal activity. The stereochemistry of 2- hydroxyheptanoic acid in verticilide was elucidated by chiral HPLC analysis of the degradation product 6 and synthetic (+) and (-)- 6. We also describe the practical total synthesis of verticilide.