Casein kinase 1 delta (CK1δ) interacts with the SNARE associated protein snapin

In this study we identified snapin as an interaction partner of the CK1 isoform delta (CK1 delta) in the yeast two-hybrid system and localized the interacting domains of both proteins. The interaction of CK1 delta with snapin was confirmed by co-immunoprecipitation. Snapin was phosphorylated by CK1 delta in vitro. Both proteins localized in close proximity in the perinuclear region, wherein snapin was found to associate with membranes of the Golgi apparatus. The identification of snapin as a new substrate of CK1 delta points towards a possible function for CK1 delta in modulating snapin specific functions. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.