Journal
JOURNAL OF CONTROLLED RELEASE
Volume 116, Issue 2, Pages 115-122Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2006.07.002
Keywords
endosomal release; epidermal growth factor; melittin; nonviral vectors; oligoethylenimine; photochemical internalization; polyplex
Ask authors/readers for more resources
Combination of the degradable polymeric gene carriers OEI-HD-1 and LT-OEI-HD-1 with an EGF targeting conjugate resulted in strongly (up to 900-fold) enhanced polyplex activity in EGF-receptor rich HUH7 hepatocellular carcinoma cells. The targeting ligand effect was DNA dose dependent, could be blocked by competitive receptor binding with unbound EGF ligand, and was not observed in receptor-negative control cells. Measures which enhance intracellular endosomal escape, either photochemically enhanced intracellular release (PCI) or the incorporation of a novel membrane-active melittin analog NMA-3, further enhanced gene transfer activity of EGF/OEI-HD-1 polyplexes. (c) 2006 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available