Journal
INTERNATIONAL JOURNAL OF PHARMACEUTICS
Volume 489, Issue 1-2, Pages 106-116Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijpharm.2015.04.059
Keywords
Topical drug delivery; Doxorubicin liposomes; Skin permeation; Cell penetrating peptides; Tape stripping; Hair follicle blocking
Categories
Funding
- NIH Minority Biomedical Research Support (MBRS)-SC1 program [SC1 GM092779-01]
- National Institute of Minority Health and Health Disparities (NIMHD) NIMHD P20 [1P20 MD006738-03]
Ask authors/readers for more resources
The objectives of this study were to develop an innovative investigative model using doxorubicin as a fluorophore to evaluate the skin permeation of nanocarriers and the impact of size and surface characteristics on their permeability. Different doxorubicin-loaded liposomes with mean particle size <130 nm and different surface chemistry were prepared by ammonium acetate gradient method using DPPC, DOPE, Cholesterol, DSPE-PEG 2000 and 1,1-Di-((Z)-octadec-9-en-1-yl) pyrrolidin-1-ium chloride (CY5)/DOTAP/1,2-dioleoyl-sn-glycero-3-phosphate (DOPA) as the charge modifier. There was minimal release of doxorubicin from the liposomes up to 8 h; indicating that fluorescence observed within the skin layers was due to the intact liposomes. Liposomes with particle sizes >600 nm were restricted within the stratum corneum. DOTAP (p < 0.01) and CY5 (p < 0.05) liposomes demonstrated significant permeation into the skin than DOPA and PEG liposomes. Tape stripping significantly (p < 0.01) enhanced the skin permeation of doxorubicin liposomes but TAT-decorated doxorubicin liposomes permeated better (p < 0.005). Blockage of the hair follicles resulted in significant reduction in the extent and intensity of fluorescence observed within the skin layers. Overall, doxorubicin liposomes proved to be an ideal fluorophore-based model. The hair follicles were the major route utilized by the liposomes to permeate skin. Surface charge and particle size played vital roles in the extent of permeation. (C) 2015 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available