Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 87, Issue 3, Pages 305-314Publisher
SPRINGER
DOI: 10.1007/s11240-006-9168-1
Keywords
auto-regulation; acidification; cell type; in vitro; Musa; Pei-Chiao; Raja
Ask authors/readers for more resources
Embryogenic cell suspensions of Musa AAA and AAB genomic groups were cultured in a maintenance culture medium for 17 generations (lasting for 238 days). The cell growth phases and medium pH changes were also observed correspondingly. Three major growth phases of AAA genomic group have been focused, namely cell releasing, proliferation and globularization phases. During almost all the subculture generations the cell stocks of AAB 'Raja' were continuously characterized by proliferating cell aggregates while the globularization phase occurred only for short duration. The medium acidity levels of the cell stocks of AAA 'Pei-Chiao' and 'Robusta' were commonly scattered in a wider range of pH 3.3-5.3, while the AAB 'Raja' were deviated in a narrow range of pH 4.0-4.6. After subculture, culture medium showed biphasic pH changes, which were drastic pH falls followed by an auto-regulated steady-state level. The steady-state pH values in each of the three growth phases (i.e. cell releasing, proliferation and globularization phases) were of 3.3-4.0, 4.0-4.8 and 4.8-5.3 respectively. Morphological bipolarity and the efficiency in the formation of somatic embryos have been thoroughly discussed. Reported research indicates that the condition of pH below 4.6 may prevent the development of embryogenic cells towards polar growth.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available