Journal
PROTEOMICS
Volume 6, Issue 24, Pages 6400-6404Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pmic.200600139
Keywords
co-detection; CyDye immunoblotting; ECL-Plex; heart; Western blotting
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The development of ECL-Plex CyDye-conjugated secondary antibodies allows the advancement of conventional Western blotting, opening up possibilities for highly sensitive and quantitative protein confirmation and identification. We report a novel proteomic method to simultaneously visualise the total protein profile as well as the specific immunodetection of an individual protein species by combining cyanine CyDye pre-labelled proteins and antibody immunoblotting. This technique proposes to revolutionise both 2-D immunoprobing and protein confirmation following MS analysis.
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